vOTU vOTU-057369

vOTU: vOTU-057369

Overview

Genome (FASTA) Annotation Track (GFF) Proteins (FASTA) Protein Annotation (TSV)

Cluster properties
vOTU ID	vOTU-057369
Number of genomes	2
Taxonomy (ICTV)	Bacteria → Firmicutes_A → Clostridia → Oscillospirales → Acutalibacteraceae → Clostridium_A → Clostridium_A leptum
Taxonomy (UHGV)	vFAM-00005 → vSUBFAM-00920 → vGENUS-01002 → vSUBGEN-11926 → vOTU-057369
Host lineage	Bacteria → Firmicutes_A → Clostridia → Oscillospirales → Acutalibacteraceae → Clostridium_A → Clostridium_A leptum
Host range breadth	Species
Lifestyle	temperate (Evidence: BACPHLIP)

Representative genome
Genome ID	UHGV-1374215
Biosample ID	N/A
Genome length (bp)	35,763 bps
CheckV completeness	100.00% (Complete)
CDS count	63
CDS density	89.56%
tRNA count	0
Integrase	No
Viral confidence	Confident

Host assignment details
Assignment method	PHIST
CRISPR-predicted host lineage	Bacteria → Firmicutes_A → Clostridia → Oscillospirales → Ruminococcaceae → Negativibacillus → Negativibacillus faecipullorum	Total connections	2	Host agreement	100
PHIST-predicted host lineage	Bacteria → Firmicutes_A → Clostridia → Oscillospirales → Acutalibacteraceae → Clostridium_A → Clostridium_A leptum	Total connections	47	Host agreement	87.23

Representative genome content

Select to view: Genome Viewer Functional Annotation

Genome view (completeness: 100)

Functional Annotation

Reference	Gene ID	Reference ID	Bit-score	E-value	Information	Category
PHROG	UHGV-1374215_1	phrog_709	117.3	8.3e-35	N/A	unknown function
eggNOG-mapper	UHGV-1374215_1	411471.SUBVAR_05858	137.0	3.7e-40	N/A	N/A
structural_annotation	UHGV-1374215_1	PF11836	N/A	N/A	N/A	N/A
PHROG	UHGV-1374215_2	phrog_88	171.7	0	major tail protein	tail
UniRef90	UHGV-1374215_2	UniRef90_A0A1C6H6F0	360.0	0	Phage major tail protein, phi13 family	N/A
eggNOG-mapper	UHGV-1374215_2	1226322.HMPREF1545_00121	190.0	0	phage major tail protein, phi13 family	N/A
NCBIFam	UHGV-1374215_2	TIGR01603.1	N/A	N/A	major tail protein	N/A
structural_annotation	UHGV-1374215_2	PF06488,TIGR01603,COG3157	N/A	N/A	N/A	N/A
PHROG	UHGV-1374215_3	phrog_78	52.2	0.000000000000012	tail terminator	connector
UniRef90	UHGV-1374215_3	UniRef90_U2SBX3	105.0	2e-27	HTH merR-type domain-containing protein	N/A
eggNOG-mapper	UHGV-1374215_3	411471.SUBVAR_05856	120.0	2.76e-34	N/A	N/A
structural_annotation	UHGV-1374215_3	PF20765	N/A	N/A	N/A	N/A
PHROG	UHGV-1374215_4	phrog_5	59.2	1.1e-16	tail completion or Neck1 protein	connector
UniRef90	UHGV-1374215_4	UniRef90_A0A8J6PH82	189.0	0	HK97 gp10 family phage protein	N/A
eggNOG-mapper	UHGV-1374215_4	411471.SUBVAR_05855	128.0	2e-36	N/A	N/A
Pfam	UHGV-1374215_4	PF04883	N/A	N/A	Bacteriophage HK97-gp10, putative tail-component	HK97-gp10_like
structural_annotation	UHGV-1374215_4	PF20039,TIGR01725,COG5005	N/A	N/A	N/A	N/A
PHROG	UHGV-1374215_5	phrog_1951	92.1	3.8e-27	head-tail adaptor	connector
UniRef90	UHGV-1374215_5	UniRef90_A0A2V2GSS5	130.0	2.65e-37	Phage head-tail adapter protein	N/A
eggNOG-mapper	UHGV-1374215_5	1226322.HMPREF1545_00118	102.0	4.91e-27	N/A	N/A
structural_annotation	UHGV-1374215_5	PF05521,TIGR01563,COG5614	N/A	N/A	N/A	N/A
PHROG	UHGV-1374215_6	phrog_2550	89.8	1.5e-26	N/A	unknown function
UniRef90	UHGV-1374215_6	UniRef90_R5V091	74.3	0.00000000000000191	Phage related protein	N/A
eggNOG-mapper	UHGV-1374215_6	1226322.HMPREF1545_00117	123.0	1.09e-35	N/A	N/A
structural_annotation	UHGV-1374215_6	PF05135,TIGR01560	N/A	N/A	N/A	N/A
PHROG	UHGV-1374215_7	phrog_10	209.2	0	major head protein	head and packaging
UniRef90	UHGV-1374215_7	UniRef90_A0A1C6H720	995.0	0	Predicted phage phi-C31 gp36 major capsid-like protein	N/A
eggNOG-mapper	UHGV-1374215_7	1226322.HMPREF1545_00116	664.0	0	phage major capsid protein, HK97 family	N/A
Pfam	UHGV-1374215_7	PF05065	N/A	N/A	Phage capsid family	Phage_capsid
Pfam	UHGV-1374215_7	PF12733	N/A	N/A	Cadherin-like beta sandwich domain	Cadherin-like
NCBIFam	UHGV-1374215_7	TIGR01554.2	N/A	N/A	phage major capsid protein	N/A
COG	UHGV-1374215_7	COG4653	N/A	N/A	Predicted phage phi-C31 gp36 major capsid-like protein	N/A
structural_annotation	UHGV-1374215_7	PF05065,MF_04119,TIGR01554,COG4653	N/A	N/A	N/A	N/A
KOfam	UHGV-1374215_8	K06904	147.8	9.94922e-44	N/A	N/A
PHROG	UHGV-1374215_8	phrog_80	171.0	0	head maturation protease	head and packaging
UniRef90	UHGV-1374215_8	UniRef90_A0A1C6H687	401.0	0	Phage prohead protease, HK97 family	N/A
eggNOG-mapper	UHGV-1374215_8	1226322.HMPREF1545_00115	251.0	0	Phage prohead protease, HK97 family	N/A
Pfam	UHGV-1374215_8	PF04586	N/A	N/A	Caudovirus prohead serine protease	Peptidase_S78
NCBIFam	UHGV-1374215_8	TIGR01543.1	N/A	N/A	HK97 family phage prohead protease	N/A
COG	UHGV-1374215_8	COG3740	N/A	N/A	Phage head maturation protease	N/A
structural_annotation	UHGV-1374215_8	PF04586,TIGR01543,COG3740	N/A	N/A	N/A	N/A
PHROG	UHGV-1374215_9	phrog_12	176.5	0	portal protein	head and packaging
UniRef90	UHGV-1374215_9	UniRef90_A0A1C6H763	807.0	0	Phage portal protein, HK97 family	N/A
eggNOG-mapper	UHGV-1374215_9	1226322.HMPREF1545_00114	561.0	0	Phage portal protein, HK97 family	N/A
Pfam	UHGV-1374215_9	PF04860	N/A	N/A	Phage portal protein	Phage_portal
NCBIFam	UHGV-1374215_9	TIGR01537.1	N/A	N/A	phage portal protein	N/A
COG	UHGV-1374215_9	COG4695	N/A	N/A	Phage portal protein BeeE	N/A
structural_annotation	UHGV-1374215_9	PF04860,MF_04120,TIGR01537,COG4695	N/A	N/A	N/A	N/A
PHROG	UHGV-1374215_10	phrog_9	296.5	0	terminase large subunit	head and packaging
UniRef90	UHGV-1374215_10	UniRef90_A0A0D8IV16	1085.0	0	Terminase	N/A
eggNOG-mapper	UHGV-1374215_10	1226322.HMPREF1545_00113	889.0	0	Phage Terminase	N/A
Pfam	UHGV-1374215_10	PF03354	N/A	N/A	Terminase large subunit, ATPase domain	TerL_ATPase
Pfam	UHGV-1374215_10	PF20441	N/A	N/A	Terminase large subunit, endonuclease domain	TerL_nuclease
COG	UHGV-1374215_10	COG4626	N/A	N/A	Phage terminase-like protein, large subunit, contains N-terminal HTH domain	N/A
structural_annotation	UHGV-1374215_10	PF20441,PF03354,MF_04013,TIGR01547,COG4626	N/A	N/A	N/A	N/A
PHROG	UHGV-1374215_11	phrog_361	70.8	2.2e-20	terminase small subunit	head and packaging
UniRef90	UHGV-1374215_11	UniRef90_A0A1C6H6I9	235.0	0	Phage terminase, small subunit	N/A
Pfam	UHGV-1374215_11	PF05119	N/A	N/A	Phage terminase, small subunit	Terminase_4
COG	UHGV-1374215_11	COG3747	N/A	N/A	Phage terminase, small subunit	N/A
structural_annotation	UHGV-1374215_11	PF05119,TIGR01558,COG3747	N/A	N/A	N/A	N/A
structural_annotation	UHGV-1374215_15	PF09274,NF041284,COG4877	N/A	N/A	N/A	N/A
PHROG	UHGV-1374215_16	phrog_36	78.3	1.2e-22	transcriptional regulator	transcription regulation
UniRef90	UHGV-1374215_16	UniRef90_A0A4Z0XVP8	102.0	2.9e-25	RNA polymerase sigma factor SigO	N/A
eggNOG-mapper	UHGV-1374215_16	883112.HMPREF9707_01621	75.1	0.00000000000000207	N/A	N/A
COG	UHGV-1374215_16	COG1191	N/A	N/A	DNA-directed RNA polymerase specialized sigma subunit	N/A
structural_annotation	UHGV-1374215_16	PF07374,MF_00674,NF008895,COG1191	N/A	N/A	N/A	N/A
PHROG	UHGV-1374215_19	phrog_25732	45.5	0.0000000000011	N/A	unknown function
UniRef90	UHGV-1374215_21	UniRef90_UPI001C3A10B1	67.0	0.00000000000709	Zinc ribbon domain-containing protein	N/A
eggNOG-mapper	UHGV-1374215_21	742738.HMPREF9460_04055	65.5	0.00000000000305	N/A	N/A
PHROG	UHGV-1374215_25	phrog_2245	95.1	7.3e-28	nucleoside triphosphate pyrophosphohydrolase	other
UniRef90	UHGV-1374215_25	UniRef90_A0A4U9RLZ8	85.5	7.24e-20	N/A	N/A
eggNOG-mapper	UHGV-1374215_25	428125.CLOLEP_01814	169.0	0	COG NOG14118 non supervised orthologous group	N/A
structural_annotation	UHGV-1374215_25	PF12643,MF_01020,TIGR03188	N/A	N/A	N/A	N/A
PHROG	UHGV-1374215_26	phrog_757	97.3	9.4e-30	DNA methyltransferase	other
UniRef90	UHGV-1374215_26	UniRef90_A0A6M3L5L0	206.0	0	Putative methyltransferase	N/A
eggNOG-mapper	UHGV-1374215_26	1226322.HMPREF1545_03521	144.0	6.67999e-41	C-5 cytosine-specific DNA methylase	N/A
structural_annotation	UHGV-1374215_26	PF00145,TIGR00675,COG0270	N/A	N/A	N/A	N/A
KOfam	UHGV-1374215_29	K01160	47.2	0.00000000000065	rusA; crossover junction endodeoxyribonuclease RusA [EC:3.1.21.10]	N/A
PHROG	UHGV-1374215_29	phrog_140	109.0	4.2e-32	RusA-like Holliday junction resolvase	DNA, RNA and nucleotide metabolism
UniRef90	UHGV-1374215_29	UniRef90_A0A1C6HG31	278.0	0	Holliday junction resolvase	N/A
eggNOG-mapper	UHGV-1374215_29	411461.DORFOR_00015	116.0	3.69e-31	Endodeoxyribonuclease RusA	N/A
Pfam	UHGV-1374215_29	PF05866	N/A	N/A	Endodeoxyribonuclease RusA	RusA
COG	UHGV-1374215_29	COG4570	N/A	N/A	Holliday junction resolvase RusA (prophage-encoded endonuclease)	N/A
structural_annotation	UHGV-1374215_29	PF05866,NF007305,COG4570	N/A	N/A	N/A	N/A
KOfam	UHGV-1374215_30	K02314	250.4	0	dnaB; replicative DNA helicase [EC:5.6.2.3]	N/A
PHROG	UHGV-1374215_30	phrog_19	241.7	0	DnaB-like replicative helicase	DNA, RNA and nucleotide metabolism
UniRef90	UHGV-1374215_30	UniRef90_A0A6N8HZR2	336.0	0	DNA helicase	N/A
eggNOG-mapper	UHGV-1374215_30	693746.OBV_27440	192.0	0	DnaB-like helicase N terminal domain	N/A
Pfam	UHGV-1374215_30	PF00772	N/A	N/A	DnaB-like helicase N terminal domain	DnaB
Pfam	UHGV-1374215_30	PF03796	N/A	N/A	DnaB-like helicase C terminal domain	DnaB_C
COG	UHGV-1374215_30	COG0305	N/A	N/A	Replicative DNA helicase	N/A
structural_annotation	UHGV-1374215_30	PF03796,PF00772,MF_04155,NF005240,COG0305	N/A	N/A	N/A	N/A
PHROG	UHGV-1374215_31	phrog_8489	193.3	0	DnaD-like helicase loader	DNA, RNA and nucleotide metabolism
UniRef90	UHGV-1374215_31	UniRef90_A0A1V4IJQ1	147.0	1.2e-39	DnaB_2 domain-containing protein	N/A
eggNOG-mapper	UHGV-1374215_31	762550.LEGAS_1609	141.0	4.93e-38	DnaD domain protein	N/A
COG	UHGV-1374215_31	COG3935	N/A	N/A	DNA replication protein DnaD	N/A
structural_annotation	UHGV-1374215_31	PF09681,TIGR01714	N/A	N/A	N/A	N/A
structural_annotation	UHGV-1374215_32	PF21715,COG1654	N/A	N/A	N/A	N/A
PHROG	UHGV-1374215_33	phrog_862	54.9	0.0000000000000016	N/A	unknown function
eggNOG-mapper	UHGV-1374215_33	1203606.HMPREF1526_00310	124.0	3.53e-33	N/A	N/A
structural_annotation	UHGV-1374215_33	PF05037,COG2965	N/A	N/A	N/A	N/A
PHROG	UHGV-1374215_40	phrog_4392	47.9	0.00000000000019	N/A	unknown function
UniRef90	UHGV-1374215_40	UniRef90_UPI000E489044	105.0	1.12e-26	membrane lipoprotein lipid attachment site-containing protein	N/A
PHROG	UHGV-1374215_41	phrog_750	114.1	5.7e-34	N/A	unknown function
eggNOG-mapper	UHGV-1374215_41	742723.HMPREF9477_00931	78.2	2.19e-17	N/A	N/A
UniRef90	UHGV-1374215_44	UniRef90_UPI001D00CEEC	103.0	2.27e-27	XRE family transcriptional regulator	N/A
eggNOG-mapper	UHGV-1374215_44	428125.CLOLEP_02737	142.0	9.80909e-44	N/A	N/A
structural_annotation	UHGV-1374215_44	TIGR03070	N/A	N/A	N/A	N/A
PHROG	UHGV-1374215_45	phrog_8	62.4	1e-17	transcriptional regulator	transcription regulation
UniRef90	UHGV-1374215_45	UniRef90_A0A855A1Q3	232.0	0	XRE family transcriptional regulator	N/A
eggNOG-mapper	UHGV-1374215_45	428125.CLOLEP_02738	226.0	0	SMART helix-turn-helix domain protein	N/A
Pfam	UHGV-1374215_45	PF01381	N/A	N/A	Helix-turn-helix	HTH_3
COG	UHGV-1374215_45	COG1396	N/A	N/A	Transcriptional regulator, contains XRE-family HTH domain	N/A
structural_annotation	UHGV-1374215_45	PF12844,NF041951,COG1396	N/A	N/A	N/A	N/A
PHROG	UHGV-1374215_46	phrog_87	78.8	5.6e-23	metallo-protease	other
UniRef90	UHGV-1374215_46	UniRef90_A7VVX5	280.0	0	ImmA/IrrE family metallo-endopeptidase	N/A
eggNOG-mapper	UHGV-1374215_46	428125.CLOLEP_02739	280.0	0	N/A	N/A
Pfam	UHGV-1374215_46	PF06114	N/A	N/A	IrrE N-terminal-like domain	Peptidase_M78
COG	UHGV-1374215_46	COG2856	N/A	N/A	Zn-dependent peptidase ImmA, M78 family	N/A
structural_annotation	UHGV-1374215_46	PF06114,COG2856	N/A	N/A	N/A	N/A
PHROG	UHGV-1374215_48	phrog_8043	51.0	0.000000000000016	N/A	unknown function
UniRef90	UHGV-1374215_48	UniRef90_A0A374JQK3	128.0	4.53e-33	HIRAN domain-containing protein	N/A
eggNOG-mapper	UHGV-1374215_48	411471.SUBVAR_05817	126.0	1.08e-32	N/A	N/A
structural_annotation	UHGV-1374215_48	PF08797	N/A	N/A	N/A	N/A
KOfam	UHGV-1374215_49	K14059	207.0	0	int; integrase	N/A
PHROG	UHGV-1374215_49	phrog_1	190.3	0	integrase	integration and excision
UniRef90	UHGV-1374215_49	UniRef90_A0A1C6FNS1	792.0	0	Integrase	N/A
eggNOG-mapper	UHGV-1374215_49	428125.CLOLEP_01837	132.0	8.39e-32	Belongs to the 'phage' integrase family	N/A
Pfam	UHGV-1374215_49	PF00589	N/A	N/A	Phage integrase family	Phage_integrase
COG	UHGV-1374215_49	COG4974	N/A	N/A	Site-specific recombinase XerD	N/A
structural_annotation	UHGV-1374215_49	PF00589,PF14659,MF_02055,TIGR02224,COG4974	N/A	N/A	N/A	N/A
PHROG	UHGV-1374215_51	phrog_13890	67.4	1.5e-19	N/A	unknown function
UniRef90	UHGV-1374215_51	UniRef90_A0A1C6F5F6	84.7	2.33e-20	Arc domain-containing protein	N/A
eggNOG-mapper	UHGV-1374215_51	742738.HMPREF9460_00716	73.9	8.45e-17	N/A	N/A
Pfam	UHGV-1374215_51	PF05534	N/A	N/A	HicB family	HicB
structural_annotation	UHGV-1374215_51	PF10723,NF010256	N/A	N/A	N/A	N/A
PHROG	UHGV-1374215_54	phrog_1281	127.4	7e-38	N/A	unknown function
UniRef90	UHGV-1374215_54	UniRef90_A0A0X8KA51	170.0	0	N/A	N/A
eggNOG-mapper	UHGV-1374215_54	1000569.HMPREF1040_1612	159.0	0	N/A	N/A
Pfam	UHGV-1374215_54	PF13274	N/A	N/A	Antitoxin SocA-like, Panacea domain	SocA_Panacea
COG	UHGV-1374215_54	COG3600	N/A	N/A	N/A	N/A
structural_annotation	UHGV-1374215_54	PF13274,COG3600	N/A	N/A	N/A	N/A
PHROG	UHGV-1374215_55	phrog_297	57.0	3.6e-16	holin	lysis
UniRef90	UHGV-1374215_55	UniRef90_A7VT88	181.0	0	Holin, phage phi LC3 family	N/A
eggNOG-mapper	UHGV-1374215_55	428125.CLOLEP_00169	181.0	0	COG COG5546 Small integral membrane protein	N/A
Pfam	UHGV-1374215_55	PF04531	N/A	N/A	Bacteriophage holin	Phage_holin_1
NCBIFam	UHGV-1374215_55	TIGR01598.1	N/A	N/A	phage holin	N/A
COG	UHGV-1374215_55	COG5546	N/A	N/A	N/A	N/A
structural_annotation	UHGV-1374215_55	COG5546	N/A	N/A	N/A	N/A
PHROG	UHGV-1374215_56	phrog_937	80.2	2.5e-23	hemolysin	other
eggNOG-mapper	UHGV-1374215_56	428125.CLOLEP_00170	223.0	0	N/A	N/A
KOfam	UHGV-1374215_57	K07273	234.2	0	acm; lysozyme	N/A
PHROG	UHGV-1374215_57	phrog_291	183.6	0	endolysin	lysis
UniRef90	UHGV-1374215_57	UniRef90_A0A1C6ERW8	422.0	0	Autolytic lysozyme	N/A
eggNOG-mapper	UHGV-1374215_57	428125.CLOLEP_00561	424.0	0	Glycosyl hydrolases family 25	N/A
Pfam	UHGV-1374215_57	PF01183	N/A	N/A	Glycosyl hydrolases family 25	Glyco_hydro_25
Pfam	UHGV-1374215_57	PF08239	N/A	N/A	Bacterial SH3 domain	SH3_3
COG	UHGV-1374215_57	COG3757	N/A	N/A	Lyzozyme M1 (1,4-beta-N-acetylmuramidase), GH25 family	N/A
structural_annotation	UHGV-1374215_57	PF01183,PF01476,TIGR02899,NF007360,COG3757,COG1388	N/A	N/A	N/A	N/A
UniRef90	UHGV-1374215_58	UniRef90_R6NDD3	98.2	1.68e-25	Glycosyl hydrolase family 25	N/A
structural_annotation	UHGV-1374215_58	PF02796,COG2771	N/A	N/A	N/A	N/A
PHROG	UHGV-1374215_59	phrog_7121	120.2	9.5e-36	tail fiber protein	tail
UniRef90	UHGV-1374215_59	UniRef90_A7VT91	302.0	0	Peptidase S74 domain-containing protein	N/A
eggNOG-mapper	UHGV-1374215_59	428125.CLOLEP_00172	311.0	0	COG COG5295 Autotransporter adhesin	N/A
Pfam	UHGV-1374215_59	PF10651	N/A	N/A	BppU N-terminal domain	BppU_N
Pfam	UHGV-1374215_59	PF13884	N/A	N/A	Chaperone of endosialidase	Peptidase_S74
PHROG	UHGV-1374215_60	phrog_1067	415.0	0	minor head protein	head and packaging
UniRef90	UHGV-1374215_60	UniRef90_A0A1C6H6B4	1006.0	0	Phage-related protein	N/A
eggNOG-mapper	UHGV-1374215_60	1123263.AUKY01000068_gene489	431.0	0	Phage minor structural protein	N/A
Pfam	UHGV-1374215_60	PF06605	N/A	N/A	Prophage endopeptidase tail	Prophage_tail
NCBIFam	UHGV-1374215_60	TIGR01665.1	N/A	N/A	phage tail spike protein	N/A
COG	UHGV-1374215_60	COG4926	N/A	N/A	Phage-related protein	N/A
structural_annotation	UHGV-1374215_60	PF14594,TIGR01665,COG4926	N/A	N/A	N/A	N/A
PHROG	UHGV-1374215_61	phrog_33	82.2	1.1e-23	distal tail protein Dit	tail
UniRef90	UHGV-1374215_61	UniRef90_A0A1C6H6D2	484.0	0	Phage-related protein	N/A
eggNOG-mapper	UHGV-1374215_61	1160721.RBI_I01575	160.0	0	Phage tail protein	N/A
COG	UHGV-1374215_61	COG4722	N/A	N/A	Phage-related protein YomH	N/A
structural_annotation	UHGV-1374215_61	PF20195,TIGR01633,COG4722	N/A	N/A	N/A	N/A
PHROG	UHGV-1374215_62	phrog_23464	262.2	0	N/A	unknown function
UniRef90	UHGV-1374215_62	UniRef90_A0A1C6H5M9	974.0	0	Phage-related minor tail protein	N/A
eggNOG-mapper	UHGV-1374215_62	1232452.BAIB02000007_gene1616	286.0	0	phage tail tape measure protein	N/A
COG	UHGV-1374215_62	COG5280	N/A	N/A	Phage-related minor tail protein	N/A
structural_annotation	UHGV-1374215_62	PF20155,TIGR01760,COG5280	N/A	N/A	N/A	N/A
PHROG	UHGV-1374215_63	phrog_3033	49.0	0.00000000000009	N/A	unknown function
eggNOG-mapper	UHGV-1374215_63	1280671.AUJH01000012_gene3142	52.4	0.0000000221	N/A	N/A

All viral genomes

ID	Representative?	Length (bps)	% GC Content	CheckV Completeness	geNomad Score
UHGV-1374215	Yes	35,763	44	100	1
UHGV-0882164	No	11,252	47	31	1